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The study of sulphonated polystyrene nanofabrics with encapsulated sensitizer
Hrdinková, Veronika ; Mosinger, Jiří (advisor) ; Vojtíšek, Pavel (referee)
The diploma thesis is dedicated to the exploitation of sulphonated polystyrene nanofibres as ion exchange nanomaterial. Ion exchange capacity of these nanomaterials has been determined by titration method and AAS. The effect of sulphonation on photophysical, photooxidative and photocytotoxic properties of sensitizer 5,10,15,20-meso- tetraphenylporphyrin (TPP) encapsulated in polystyrene nanofibres has been studied as well. Properties of TPP have been examined with time-resolved spectroscopy, photooxidation of uric acid as substrate and bactericidal tests on Escherichia coli DH5α with pGEM11Z plasmid. It has been discovered that following the sulphonation of the nanofabrics, the encapsulated sensitizer is partly present even in aggregation form. The polystyrene nanofabrics with encapsulated sensitizer keep its bactericidal efficiency also after sulphonation.
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The study of sulphonated polystyrene nanofabrics with encapsulated sensitizer
Hrdinková, Veronika ; Mosinger, Jiří (advisor) ; Vojtíšek, Pavel (referee)
The diploma thesis is dedicated to the exploitation of sulphonated polystyrene nanofibres as ion exchange nanomaterial. Ion exchange capacity of these nanomaterials has been determined by titration method and AAS. The effect of sulphonation on photophysical, photooxidative and photocytotoxic properties of sensitizer 5,10,15,20-meso- tetraphenylporphyrin (TPP) encapsulated in polystyrene nanofibres has been studied as well. Properties of TPP have been examined with time-resolved spectroscopy, photooxidation of uric acid as substrate and bactericidal tests on Escherichia coli DH5α with pGEM11Z plasmid. It has been discovered that following the sulphonation of the nanofabrics, the encapsulated sensitizer is partly present even in aggregation form. The polystyrene nanofabrics with encapsulated sensitizer keep its bactericidal efficiency also after sulphonation.
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The study of photodynamic phenomenon on melanom cell lines
KOLÁČKOVÁ, Zdeňka
Photodynamic therapy (PDT) is a medical method combining the use of photodynamic active substance and light in presence of oxygen. It extends the treatment possibilities of tumor and non-tumor disorders. It complements surgical treatment, radiotherapy, chemotherapy and immunotherapy. An increased accumulation of photosensitive substance in pathological focus is the base of PDT. Subsequent irradiation by light of suitable wavelenght evokes photodynamic reactions leading to formation of reactive oxygen species and to biological answer leading to tumor cells damage. The final effect depends on sensitizer type, its concentration in target tissue and on used source of radiation. Aim of the thesis is to prove photodynamic properties of newly developed photodynamic active substance phtalocyanin CIAIPcS2 and possibilities of its usage to induce photodynamic phenomenon in melanom cells. Owing to absorption of light in sensitizer the formation of excited states happens, and then the excited form of sensitizer reacts directly with substrate. During this reaction free radicals of substrate form or transfer of energy from sentitizer to oxygen and formation of highly reactive singlet oxygen happen. Free radicals, especially radicals of lipid components of cell membranes, are the major cause of tumor destruction. Fruitfulness of PDT depends not only on type of sensitizer and level of oxygen in tumor cell but also on used light source. Luminiscence diodes (LEDs) were used as the source of light. Formation of ROS after PDT was detected with the help of fluorescent molecular probe CM-H2DCFDA on spectrofluorimeter Synergy HT and on fluorescent microscope Olympus IX 81 with image analysis. According to our results the production of ROS depends on concentration of sensitizer CIAIPcS2 and the radiation dose. We proved that the sensitizer CIAIPcS2 is suitable photodynamic active substance and evokes photodynamic phenomenon in tumor cells.
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